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7.3 – Pathways of Infection

 How Disease Becomes Established

Pathogens use various pathways to infect seeds. To understand the disease cycle of seedborne pathogens—or the pathway of seed infection—it’s important to first familiarize yourself with seed and flower anatomy, as well as the terminology related to infection sites. Pathogen pathways can occur at all stages of seed development.  This illustration highlights the key anatomy of flower structures.

Female parts include pistil (stigma, style, ovary), ovules and nectary. Male parts include pollen, stamen (anther, filament), pollen tube growing down style to ovules.
Diagram of female and male flower anatomy. Image from: Home – Science Sauce

 


An explanation of flower anatomy and fertilization can be found here:

 

As explained in the video above, seed development occurs in the flower’s ovary. Pathogen infection can happen during any of the three key stages of seed development include 1) anthesis (flowering) and pollination (penetration through floral parts), 2) seed development (formation) and 3) seed maturation.  We’ll dive into more details about these stages next.

After pollination and fertilization, seed development begins immediately. The seed becomes the primary “sink” for nutrients. Once the ovule is fertilized the seeds begin to develop and mature through three development stages: cell division (histo-differentiation), physiological maturity (cell expansion), and maturation drying as illustrated in this diagram.  At each of these stages, pathogens can be introduced.

Stage I shows increasing grain weight. Stage 2 shows max fresh weight, water weight and dry weight. Water weight begins decreasing midway of this stage. Stage 3 shows dry weight holding steady, while water weight dramatically decreases to near zero and fresh weight shows a slight decline.
Illustration of stages of seed development.  Seed Development and Maturation.  M. Dadlani, D. K. Yadava (eds.), Seed Science and Technology, https://doi.org/10.1007/978-981-19-5888-5_2

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Two seeds, showing the scar where they detached from mother plant.

Photographs showing the funiculus and what the seed looks like after the funiculus detaches. Photos from https://propg.ifas.ufl.edu/04-seeds/01-development/07-seedsdevelopment-funiculus.html

 

How do Seedborne Pathogens Enter Developing Seeds?

Click on each of these pathogen infection pathways which occur during anthesis and pollination:

Infection During Anthesis and Pollination

Anthesis refers to the opening of the flower bud and the development of a fully functional flower.  The flower becomes ‘functional’ when the style is extended far beyond the upper perianth.  In this 5 mins video Laura introduces this topic and includes photo examples.

Infection of Seed During Seed Development and Maturation

Laura will introduce the concept of seeds becoming infected during seed development/formation and maturity stages in this short video clip.

 

 

 

 


Pathway Examples During Seed Development and Maturation

Pathogen entry during seed development and maturation can occur via:

  • Systemic infection through the mother plant: e.g., dwarf bunt in wheat and black rot in cabbage
  • Infection through natural openings: e.g., through the hilum or funiculus in bacterial diseases
  • Direct penetration of seed or caryopsis: e.g., Fusarium head blight/scab in wheat
  • Invasion from pods or fruits: e.g., bacterial fruit blotch, bacterial canker
  • External Contamination

In this video (8 mins), Laura describes invasion of pathogens through natural openings, wounds and systemic infection via mother plants.


Summary

Throughout seed growth and development, seeds are exposed multiple times to pathogens. Whether disease symptoms appear depends on environmental conditions and the presence of the right pathogen in the right location. Pathogens follow multiple infection pathways, from systemic infection of mother plants and vector transmission through infected pollen to fungal invasion via the stigma and style. Pathogens can infect internal seed tissues, like the embryo, or only contaminate the seed surface without infecting seed tissue.

 

Review

Work through these interactive questions to review the important concepts discussed.

 

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License

Seed Conditioning 107 - Seedborne Disease and Treatment Copyright © 2024 by Laura Pottorff; Tamla Blunt; Rick Novak; and Deana Namuth-Covert. All Rights Reserved.