Vegetables and Pulses
Pea PI 180693 – Root Rot Resistance
NPGS ACCESSION PI 180693 LAUNCHES MULTIPLE DISEASE RESISTANCE BREEDING IN PEA
Clarice J. Coyne; Marilyn L. Warburton; and Gayle M. Volk
USDA-ARS Plant Germplasm Introduction and Testing Research Unit, 201 Clark Hall, Washington State University, Pullman, Washington 99164.
USDA-ARS Plant Germplasm Introduction and Testing Research Unit, 201 Clark Hall, Washington State University, Pullman, Washington 99164.
USDA-ARS National Laboratory for Genetic Resources Preservation, 1111 S Mason St, Fort Collins, Colorado 80521.
Corresponding author: coynec@wsu.edu
OUTLINE
1. SUMMARY
Aphanomyces root rot, a devastating oomycete (a fungal-like protist) pathogen, causes up to 100% yield loss in peas. Pea (Pisum sativum L.) accession PI 180693, a landrace maintained by the U.S. National Plant Germplasm System (NPGS) unit in Pullman, Washington, was found to have resistance to this and other diseases. Genetic resistance from this accession was bred into improved cultivated backgrounds and released to the breeding community for both Aphanomyces root rot and Fusarium root rot. Genetic mapping populations designed to pinpoint chromosomal regions holding genes for disease resistance were developed from PI 180693 and PI 180693-derived lines; these populations enabled the discovery of major resistant genes for both Aphanomyces and Fusarium root rots.
The goal was to release pea germplasm with tolerance or resistance to Aphanomyces root rot, Fusarium root rot, and Fusarium wilt.
Download a printable fact sheet by clicking the image below.
2. PROBLEMS ADDRESSED
Peas grown in the field are particularly susceptible to fungal and oomycete rots, especially those attacking roots such as Aphanomyces root rot and Fusarium root rot. These diseases can cause total yield loss, and it is recommended to take peas out of rotation for 4 to 8 years when a field is badly infected. This greatly diminishes the value of peas in crop rotations with cereals, presenting a major problem for the many growing areas in the United States that have few other economically feasible rotation options available.
3. SOLUTIONS DEVELOPED
PI 180693, a pea landrace accession maintained by the NPGS genebank unit in Pullman, Washington, was identified as a source of resistance to Aphanomyces root rot by Kraft (1992). A succession of improved breeding lines for the U.S. dry and fresh pea markets were released containing genes from PI 180693 conferring resistance to Aphanomyces eutiches root rot as well as resistance for Fusarium solani root rot (Kraft, 1992; Kraft & Coffman, 2000; Coyne et al., 2008; McGee et al., 2012) and Fusarium wilt (Kraft & Coffman, 2000).
A major gene providing increased resistance to Aphanomyces root rot was identified from NPGS genebank accession PI 180693 and verified in new lines bred from parent PI 557501 (derived from PI 180693) and a susceptible parent (Hamon et al., 2011; 2013; Lavaud et al., 2015). The first high density SNP map of pea was developed from PI 180693 and used for fine mapping resistance and identifying candidate genes (Boutet et al., 2016; Kälin et al., 2023; 2024). Most recently, PI 180693-derived genetic stocks were used to develop Aphanomyces resistance lines (Leprévost et al., 2023). An important gene providing increased resistance to Fusarium root rot was identified by Coyne et al. (2015) and confirmed in a second population (Coyne et al., 2019). The identification of the genes responsible for Aphanomyces and Fusarium root rot tolerances and the creation of tolerant breeding lines paved the way for release of the first partially resistant cultivars by public and private pea breeding programs.
Collaborators involved in developing solution:
- Marie-Laure Pilet-Nayel, Institut National De La Recherche Agronomique, Paris, France
- Niklaus Grünwald, USDA-Agricultural Research Service, Corvallis, Oregon, USA
- John Kraft and Lyndon Porter, USDA-Agricultural Research Service, Prosser, Washington, USA
- Clarice Coyne, Marilyn Warburton, and Rebecca McGee, USDA-Agricultural Research Service, Pullman, Washington, USA
- Fred Muehlbauer, Washington State University, Pullman, Washington, USA
4. IMPACT
Public and private breeders relied on germplasm derived from the NPGS accession PI 180693 to improve partial resistances to Aphanomyces root rot and Fusarium root rot in pea cultivars. Of the 55,000 pea seed packets shipped from the NPGS genebank unit in Pullman, Washington in the last 10 years, PI 180683 was the second most requested accession.
5. GERMPLASM
PI 180693, a pea landrace from Germany named ‘Hohenheimer Pink-Flowered’, was donated to the NPGS in 1949 and is maintained by the USDA Plant Germplasm Introduction and Testing Research Unit in Pullman, Washington. Learn more about this important accession at GRIN-Global: PI 180693 and PI 180693 PSP.
Releases derived from PI 180693 include: PI 557501, PI 660729, PI 660730, PI 660731, PI 660732, PI 660733, PI 660734, PI 660735, PI 660736, W6 26740, W6 26743, W6 26745.
6. REFERENCES
Boutet G, Alves Carvalho S, Falque M, Peterlongo P, Lhuillier E, Bouchez O, Lavaud C, Pilet-Nayel M-L, Rivière N, Baranger A. 2016. SNP discovery and genetic mapping using genotyping by sequencing of whole genome genomic DNA from a pea RIL population. BMC Genomics 17:1-14. https://doi.org/10.1186/s12864-016-2447-2
Coyne CJ, Pilet-Nayel M-L, McGee RJ, Porter LD, Smykal P, Grünwald NJ. 2015. Identification of QTL controlling high levels of partial resistance to Fusarium solani f. sp. pisi in pea. Plant Breeding 134:446-453. https://doi.org/10.1111/pbr.12287
Coyne CJ, Porter LD, Inglis DA, Grünwald NJ, McPhee KE, Muehlbauer FJ. 2008. Registration of W6 26740, W6 26743 and W6 26745 pea germplasm resistant to Fusarium root rot. Journal of Plant Registrations 2:137-139. https://doi.org/10.3198/jpr2007.12.0674crg
Coyne CJ, Porter LD, Boutet G, Ma Y, McGee RJ, Lesné A, Baranger A, Pilet-Nayel M-L. 2019. Confirmation of Fusarium root rot resistance QTL Fsp-Ps 2.1 of pea under controlled conditions. BMC Plant Biology 19:1-8. https://doi.org/10.1186/s12870-019-1699-9
Hamon C, Baranger A, Coyne CJ, McGee RJ, Le Goff I, L’anthoëne V, Esnault R, Rivière JP, Klein A, Mangin P, McPhee KE, Roux-Duparque M, Porter L, Miteul H, Lesné A, Morin G, Onfroy C, Moussart A, Tivoli B, Delourme R, Pilet-Nayel M-L. 2011. New consistent QTL in pea associated with partial resistance to Aphanomyces euteiches in multiple French and American environments. Theoretical and Applied Genetics 123:261-281. https://doi.org/10.1007/s00122-011-1582-z
Hamon C, Coyne CJ, McGee RJ, Lesné A, Esnault R, Mangin P, Hervé M, Le Goff I, Deniot G, Roux-Duparque M, Morin G. 2013. QTL meta-analysis provides a comprehensive view of loci controlling partial resistance to Aphanomyces euteichesin four sources of resistance in pea. BMC Plant Biology 13:1-19. https://doi.org/10.1186/1471-2229-13-45
Kälin C, Piombo E, Bourras S, Brantestam AK, Dubey M, Elfstrand M, Karlsson M. 2024. Transcriptomic analysis identifies candidate genes for Aphanomyces root rot disease resistance in pea. BMC Plant Biology 24:1-16. https://doi.org/10.1186/s12870-024-04817-y
Kälin C, Kolodinska Brantestam A, Arvidsson AK, Dubey M, Elfstrand M, Karlsson M. 2023. Evaluation of pea genotype PI180693 partial resistance towards aphanomyces root rot in commercial pea breeding. Frontiers in Plant Science 14:1114408. https://doi.org/10.3389/fpls.2023.1114408
Kraft JM, 1992. Registration of 90-2079, 90-2131, and 90-2322 pea germplasms. Crop Science 32:1076. https://doi.org/10.2135/cropsci1992.0011183X003200040063x
Kraft JM, Coffman VA. 2000. Registration of 97-363, 97-2170, and 97-2162 Pea Germplasms. Crop Science 40:303-303. https://doi.org/10.2135/cropsci2000.0008rgp
Lavaud C, Lesné A, Piriou C, Le Roy G, Boutet G, Moussart A, Poncet C, Delourme R, Baranger A, Pilet-Nayel M-L, 2015. Validation of QTL for resistance to Aphanomyces euteiches in different pea genetic backgrounds using near-isogenic lines. Theoretical and Applied Genetics 128:2273-2288. https://doi.org/10.1007/s00122-015-2583-0
Leprévost T, Boutet G, Lesné A, Rivière J-P, Vetel P, Glory I, Miteul H, Le Rat A, Dufour P, Regnault-Kraut C, Sugio A, Lavaud C, Pilet-Nayel M-L. 2023. Advanced backcross QTL analysis and comparative mapping with RIL QTL studies and GWAS provide an overview of QTL and marker haplotype diversity for resistance to Aphanomyces root rot in pea (Pisum sativum). Frontiers in Plant Science 14:1189289. https://doi.org/10.3389/fpls.2023.1189289
McGee RJ, Coyne CJ, Pilet-Nayel ML, Moussart A, Tivoli B, Baranger A, Hamon C, Vandemark G, McPhee K. 2012. Registration of pea germplasm lines partially resistant to aphanomyces root rot for breeding fresh or freezer pea and dry pea types. Journal of Plant Registrations 6:203-207. https://doi.org/10.3198/jpr2011.03.0139crg
7. CHAPTER INFORMATION
Citation: Coyne CJ, Warburton ML, Volk GM. 2024. Pea PI 180693 – Root Rot Resistance. In: Volk GM, Chen K, Byrne P (Eds.) Plant Genetic Resources: Success Stories. Fort Collins, Colorado: Colorado State University. Date accessed. Available from https://colostate.pressbooks.pub/pgrsuccessstories/chapter/pea-pi-180693-root-rot-resistance/
Content originally submitted: February 27, 2024
Date of publication: May 20, 2024
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